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Some patients achieve their weight loss goals and maintain results on the starting dose long-term, while others develop tolerance or require higher doses for continued appetite suppression

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Mutation of the GLD-1 and POS-1 binding sites in the endogenous glp-1 gene To understand the physiological impacts of mutating the GLD-1 and POS-1 binding sites in the endogenous glp-1 locus, we used CRISPR-Cas9 mutagenesis to make precise mutations in an OLLAS-tagged glp-1 strain corresponding to the variants we previously made in our reporter strains ( Fig